JRC GMO-Amplicons | EURL GMFF

Record ID:	FR667077.1
Database:	nt	Go to original record
Description:	Gymnocalycium schickendantzii chloroplast DNA containing trnT-trnL-trnF loci, specimen voucher HA88-123-1
Taxon:	Gymnocalycium schickendantzii
Original size:	850 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	175	717	+	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aatgaatcaa
101   aatgggcaat cctgagccaa ctcctttcaa aaaaaagcaa aaaataaggg
151   ttcagaaagc aagaatcaaa gaaaaggaaa ggtgcagaga ctcaaaggaa
201   gttgttctaa caaatgaggt tgactgtgct gtgttcttat cagaattctt
251   ccatcaaaac tccaattcaa aaaaagggta aagcatatac gtagtgaact
301   atatccaatg atgaatgaca acccgtttat gtatatatct aactatatat
351   aatcagataa tatgaaaaaa tagataattc tatcgattag aatctgaaat
401   gaaaaaatgg aagaactgat gaatcggacg agaatgaaga tagagtcccg
451   ttctacatgt caatactgac aacaaagaaa tttcgagtaa gaggaaaatc
501   cgtcgacttt taaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details