JRC GMO-Amplicons | EURL GMFF

Record ID:	GU818009.1
Database:	nt	Go to original record
Description:	Helichrysum stoechas isolate 1164 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Helichrysum stoechas
Original size:	907 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	528	+	528 bp

Amplicon:

528 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aatcaaaagg ataggtgcag agactcgatg gaagctgttc
201   taacaaatgg agttgattgt cttacattgg tagagaaatc cttctatgga
251   aacttcagaa aagatgaagg ataaacctgt atacataata gagatacaga
301   agaattgata tgaatcgatt ccatattgaa gaaagaatcg aatatcgaat
351   attcattgat caaaccattc actccataat ctgatagatc ttttgaagaa
401   ctgattaatc ggacgagaat aaagatagag tcccgttcta catgtcaata
451   ccggcaacaa tgaaatttat agtaagagga aaatccgtcg atttcaaaaa
501   tcatgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	528	509	2.37e-08

Match details