JRC GMO-Amplicons | EURL GMFF

Record ID:	HM042585.1
Database:	nt	Go to original record
Description:	Spermacoce princeae voucher Luke & Luke 8371 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Spermacoce princeae
Original size:	1769 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	796	1382	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccactgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctatttt ccgaaaacaa aaaaaagggg
151   ttcataaagt gaaaaagggg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg agttggatgc gttagtcgat aagtctttcc aggaaaaatt
251   ccttaagtag ttaaggataa agcgaaggat aaacgtatat acatacgtag
301   tgaatagcat attaaatgag tactgacagc ccaaactaat ctctattttt
351   tctatgaaaa aagaaagaat tgttgttaat agattctatg tttaagaaag
401   aatcgaatat gcattgatca aatgattcac tccatagtct gatagatctt
451   ttcacgaact gattaatcag acgagaataa agatagagtc ccattttaca
501   tgtcaattcc ggcaacaatg aaatttatag ttaataggaa aatccgtcga
551   cttttaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details