JRC GMO-Amplicons | EURL GMFF

Record ID:	AY305826.1
Database:	nt	Go to original record
Description:	Manglietia conifera subsp. chingii trnL, trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Magnolia conifera var. chingii
Original size:	982 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	419	982	-	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgtgttcag aaaacaaggg ttcagaaagc
151   gagaatcaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagtta ctgcattggt agaggaatcg aatccttcta tcgaaactac
251   agaaaagatg accctgtata cgtacgtata catactgaaa tatcaaataa
301   ttaatcacga ctcgaatcct tattttttta tatgaaaaat ttaagaatta
351   ttgtgaatcg attccaagtt gaaggaagaa tcgaatattc agtgatcaaa
401   tcattcactc ccgagtctga tagatctttt gaagaactga ttaatcggac
451   gagaataaag atagagtccc attctacatg tcaatacaga caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt tagaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details