Match details

Record ID: HM439359.1
Database: nt Go to original record
Description: Plant binary vector pXNS1pat-Strep, complete sequence.
Taxon: Plant binary vector pXNS1pat-Strep
Original size: 5316 bp

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 5068 5263 + 196 bp

Amplicon: 196 bp
1     gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagaa
51    tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca
101   tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
151   tgtgatatct ccactgacgt aagggatgac gcacaatccc actatc 

View other 185 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.45e-06
primerB 100.0 20 bp 0 0 196 177 9.33e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.