JRC GMO-Amplicons | EURL GMFF

Record ID:	GU943568.1
Database:	nt	Go to original record
Description:	Agalinis tenuifolia isolate 46ATUMD tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis tenuifolia
Original size:	1530 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	627	1186	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaaaca agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ttgcgctgat agaggaatga aacttcagaa aggatgaagg
251   ataaacgtat ctattgaata ctatatcaaa atcaaatgat tgattaatga
301   tggcccaaat ctctatctgt atttttgaat acgaaaagtg gaagaattgg
351   tgtgaatttc ttccataaag aagattcatt catcaaatca ttcactccat
401   agtccgatag atctttttta aatttaaaag atctgattaa tcggacgaga
451   ataaagatag agtcccattc tacatgtcaa taccggcaac aatgaaattt
501   atagtaaaag gaaaatccgt cgacttttaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details