JRC GMO-Amplicons | EURL GMFF

Record ID:	AY305820.1
Database:	nt	Go to original record
Description:	Magnolia officinalis subsp. biloba trnL, trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Magnolia officinalis subsp. biloba
Original size:	983 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	419	983	-	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgtgttcag aaaacaaggg ttcagaaagc
151   gagaatcaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcattgg tagaggaatc gattccttct atcgaaacta
251   cagaaaagat gaccctgtat acgtacgtat acatactgaa atatcaaata
301   attaatcacg actcgaatcc ttattttttt atatgaaaaa tttaagaatt
351   attgtgaatc gattccaagt tgaaggaaga atcgaatatt cagtgatcaa
401   atcattcact cccgagtctg atagatcttt tgaagaactg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaatacag acaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttagaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details