JRC GMO-Amplicons | EURL GMFF

Record ID:	GU192019.1
Database:	nt	Go to original record
Description:	Anatherostipa orurensis voucher Peterson at al. 12987 (MO) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Anatherostipa orurensis
Original size:	978 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	599	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagc ggttctcgaa
151   ctagaggata ggtgcagaga ctcaatggaa gctgttctaa cgaatcgagt
201   taattacttg tgttggtagt ggaattcctt ctaaattaga gagagagggg
251   ctttatacat ctaataaaca cgtatagata ctgacatagc aaacgattaa
301   tcacagaacc catatcataa tataggttct ttattctttt ttagaatgaa
351   ataaggaatg attatgaaat aaaaaattca taattttttt cgaattattg
401   tgaattcatt ccaatcgaat attgagtaat caaatccttc aattcatagt
451   tttcgagatc ttttaaaaag tggattaatc ggacgaggac aaagagagag
501   tcccattcta catgtcaata ctggcaacaa tgaaatttct agtaaaagga
551   aaatccgtcg actttataag ttgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details