JRC GMO-Amplicons | EURL GMFF

Record ID:	GU192016.1
Database:	nt	Go to original record
Description:	Ortachne erectifolia voucher Wood 5263 (MO) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Lorenzochloa erectifolia
Original size:	976 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	598	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    actgctaagt ggtaacttcc aaattcagag aaaccctgga attaaaaaag
101   ggcaatcctg agccaaatcc gtgttttgag aaaacaagcg gttctcgaac
151   tagaggatag gtgcagagac tcaatggaag ctgttctaac gaatcgattt
201   aattacttgt gttggtagtg gaattccttc taaattagag agagaggggc
251   tttatacatc taataaacac gtatagatac tgacatagca aacgattaat
301   cacagaaccc atatcataat ataggttctt tattcttttt tagaatgaaa
351   taaggaatga ttatgaaata aaaaattcag aatttttttc gaattattgt
401   gaattcattc caatcgaata ttgagtaatc aaatccttca attcatagtt
451   ttcgagatct tttaaaaagt ggattaatcg gacgaggaca aagagagagt
501   cccattctac atgtcaatac tggcaacaat gaaatttcta gtaaaaggaa
551   aatccgtcga ctttataagt tgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details