JRC GMO-Amplicons | EURL GMFF

Record ID:	GU192006.1
Database:	nt	Go to original record
Description:	Austrostipa elegantissima voucher Peterson 14289 (MO) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Austrostipa elegantissima
Original size:	978 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	598	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagt ggttctcgaa
151   ctagaggata ggtgcagaga ctcaatggaa gctgttctaa cgaatcgagt
201   taattacttg tgttggtagt ggaattcctt ctaaattaga gagagaaggg
251   ctttatacat ctaataaaca cgtatagata ctggcatagc aaacgattaa
301   tcacagaacc catatcataa tataggttct ttattctttt ttagaatgaa
351   attaggaaag attatgaaat aaaaaattct gaattttttt gaattattgt
401   gaattcattc caatcgaata ttgagtaatc aaatccttca attcatagtt
451   ttcgagatct tttaaaaagt ggattaatcg gacgaggaca aagagagagt
501   cccattctac atgccaatac tggcaacaat gaaatttcta gtaaaaggaa
551   aatccgtcga ctttataagt tgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details