JRC GMO-Amplicons | EURL GMFF

Record ID:	GU445347.1
Database:	nt	Go to original record
Description:	Lilium formosanum voucher Boufford, E.W. Wood & C.F. Hsieh #19253 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Lilium formosanum
Original size:	843 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	609	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgcaaag tgataacttc caaatccaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tatgaaacta caataaaaaa aagataggtg cagagactca
201   atggaagctg ttctaacgaa tgaagttgat tacgttgcgc tggtagccgg
251   aattcttcta tcaaaatgac agagaggatg cccgccctat atacgtatat
301   atacatactg acatagtaaa cgattgatta atcgcggccc gaatccatta
351   taatatatat atatatgaaa aatttaagag ttattgtaaa tctattccat
401   attccaatca gagtttaagg aagaatcgaa tattcagtga tcaaattatt
451   cattccagag tctgtataga tcttttaaaa actgattatt cggacgagaa
501   taaagagaga gtcccattct acgtgtcaat accgacaaca atgaaattta
551   tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details