JRC GMO-Amplicons | EURL GMFF

Record ID:	GU445346.1
Database:	nt	Go to original record
Description:	Lilium brownii voucher H. Lam #20 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Lilium brownii
Original size:	843 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	607	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacctgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tataaaacta caataaaaaa aagataggtg cagtgaccca
201   atggaagctg ttctaacgaa tgaagttgat tacgttgcgc tggtagccgg
251   aattcttcta tcaaaatgac agagaggatg cccgccctat atacgtatat
301   atacatactg acatagtaaa cgattgatta atcgcggccc gaatccatta
351   taatatatat atatgaaaaa tttaagagtt attgtaaatc tattccatat
401   tccaatcaaa gtttaaggaa ggatcgaata ttcagtgatc aaattattca
451   ttccagagtc tgtatagatc ttttaaaaac tgattattcg gacgagtata
501   aagagagagt cccattctac gtgtcaatac cgacaacaat gaaatttata
551   gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details