JRC GMO-Amplicons | EURL GMFF

Record ID:	GU445344.1
Database:	nt	Go to original record
Description:	Cardiocrinum giganteum voucher 3689 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Cardiocrinum giganteum
Original size:	847 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	611	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacctc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagctgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   atccttctat caaaattacg gagaggatgc ccgccctata tacgtatata
301   tacatactga catagtaaac gattgattaa tcacggcccg aatccattat
351   ataatatata tatatatgaa aaatttaaga gttattgtaa atccattcca
401   tattccaatc aaagtttaag gaagaatcaa atattcagtg atcaaattat
451   tcgttccaga gtctgtatag atctttttaa aaactgatta ttcggacgag
501   aataaagaga gagtcccatt ttacgtgtca ataccgacaa caatgaaatt
551   tatagtaaaa ggaaaatccg tcgactttat aagtcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details