JRC GMO-Amplicons | EURL GMFF

Record ID:	GU445341.1
Database:	nt	Go to original record
Description:	Aristolochia kaempferi f. heterophylla voucher 1980 Sino-Amer Exped #1121 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Aristolochia heterophylla
Original size:	955 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	529	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaaaaag gataggtgca
151   gagactcaac ggaagctgtt ctaacgaatg gggttgactt tactacattg
201   gtagaagaat ccttctatcg aaactccaga gtgggatgac cctagatatg
251   tacgtatacg tactgaaata tcaaagatta atcacgaccc gaatccatat
301   tttttttttc taaaaatttc agaagcagaa ggaagaatcg aatagtcagg
351   gatcaaatcg actatcacac cagagtctga tagatctttt taaggattga
401   ttaatcgagt gagacgagaa taaagataga gtcccattct acatgtcaat
451   actgacaaca atgaaattta tagtaagagg aaaatccgtc gactttagaa
501   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details