JRC GMO-Amplicons | EURL GMFF

Record ID:	GU445340.1
Database:	nt	Go to original record
Description:	Aristolochia debilis tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Aristolochia debilis
Original size:	1031 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	592	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttgaa aaaaaaaaaa aaagttcaga
151   ataaagaata aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaataaa tggggttgac tttactttac tacataggtg gaagaatcct
251   tctatcaaaa ctccagagtg ggatgaccct agatctatat atatctatat
301   atgtacgtat atgtactgaa atatcaaaga ttaatcacga cccgaatcta
351   tatatatata tttttctcta tggtaaaaat ttcagaagca gaaggaagaa
401   tcaaatagtc agtgatcaaa tcgactatca caccagagtt tgatagatct
451   ttttaaagat tgattaatcg ggcgagacga gaataaagat agagtcccat
501   tctacatgtc aatactgaca acaatgaaat ttctagtaag aggaaaatcc
551   gtcgacttta gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details