JRC GMO-Amplicons | EURL GMFF

Record ID:	GU445338.1
Database:	nt	Go to original record
Description:	Aristolochia debilis voucher K. Yonekura #97283 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Aristolochia debilis
Original size:	1035 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	597	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttgaa aaaaaaaaaa aaaagttcag
151   aataaagaat aaaaaaaagg ataggtgcag agactcaacg gaagctgttc
201   taacaaataa atggggttga ctttacttta ctacataggt ggaagaatcc
251   ttctatcaaa actccagagt gggatgaccc tagatctata tatatctata
301   tatgtacgta tatgtactga aatatcaaag attaatcacg acccgaatct
351   atatatatat atatattttt ctctatgata aaaatttcag aagcagaagg
401   aagaatcaaa tagtcagtga tcaaatcgac tatcacacca gagtttgata
451   gatcttttta aagattgatt aatcgggcga gacgagaata aagatagagt
501   cccattctac atgtcaatac tgacaacaat gaaatttcta gtaagaggaa
551   aatccgtcga ctttagaaat cgtgagggtt caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details