JRC GMO-Amplicons | EURL GMFF

Record ID:	GU445336.1
Database:	nt	Go to original record
Description:	Aristolochia championii voucher HK 38319 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Aristolochia championii
Original size:	954 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	529	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gaacttgatt ggattgagcc ttggtttcca
51    aacctactaa gtggtaactt ccaaattcag agaaaccctg gaattaaaaa
101   tgggcaatcc tgagccaaat cctgttttcw gaaacnaaaa ggataggtgc
151   agagactcaa cggaagctgt tctaacgaat ggggttgact ttactacatt
201   ggtagaagaa tccttctatc gaaactccag agtgggatga ccctagatat
251   gtacgtatac gtactgaaat atcaaagatt aatcacgacc cgaatccata
301   tttttttttc taaaaatttc agaagcagaa ggaagaatcg aatagtcagt
351   gatcaaatcg actatcacac cagagtctga tagatctttt taaggattga
401   ttaatcgagt gagacgagaa taaagataga gtcccattct acatgtcaat
451   actgacaaca atgaatttta tagtaagagg aaaatccgtc gactttagaa
501   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details