JRC GMO-Amplicons | EURL GMFF

Record ID:	GU445335.1
Database:	nt	Go to original record
Description:	Aristolochia californica voucher 19176 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Aristolochia californica
Original size:	995 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	565	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaaaaag gataggtgca
151   gagactcaac ggaagctgtt ctaacgaatg gggttgactt tactacattg
201   gtagaagaat ccttctatcg aaactccaga gtgggatgac cctagatatg
251   tacgtatacg tactgaaata tcaaagatta atcacgaccc gaatccatat
301   ttttttttct aaaaatttca gaataataat ttcagaagca gaaggaagaa
351   tcgaatagtc agtgatcaaa tcgactatca caccagagtc tgatagatct
401   ttttaaggat gtctgataga tctttttaag gattgattaa tcgagtgaga
451   cgagaataaa gatagagtcc cattctacat gtcaatactg acaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttagaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details