JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ852530.1
Database:	nt	Go to original record
Description:	Omiltemia filisepala trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Omiltemia filisepala
Original size:	1678 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	724	1301	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa agattcagaa
151   agtgaaaaaa aggataggtg cagagactca acggaagctg ttctaacaaa
201   tggagttggc tgcgttagta gagaaatctt tccatcgaaa atgaaaattc
251   agaaagaatg aagtgaagga taaacgtata tacatacgta ttgaatacta
301   tatcaaatga ttcatgacga cccgactgaa tctgtatttt tatatatgaa
351   aaacggaaga attggtgtga atagattcca cattgaagaa ataatcgaat
401   attcattgat caaacgattc cctccatagt ctgatagatc ttttcaagaa
451   ctgattaatc ggacgagaat aaagatagag tcccgttcta catgtcaatg
501   ccggcaacaa tgaaatttat agtaagagga aaatccgtcg actttaaaaa
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details