JRC GMO-Amplicons | EURL GMFF

Record ID:	FN386357.1
Database:	nt	Go to original record
Description:	Rytigynia sp. Ranirison 624 trnT-trnL IGS(partial), tRNA-Leu gene and trnL-trnF IGS(partial), specimen voucher G:Ranirison 624
Taxon:	Rytigynia sp. Ranirison 624
Original size:	1642 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	775	1334	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa tggaagttgt tctaacaaat
201   ggagttgtct gcgttagtaa agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactatatc aaatgattaa tgacgcctcg
301   actgaatccg tattttttaa ttttttatat gaaaacggaa gaattggtgt
351   gaatagattc cacattgaag aaagaatcga atattcattg attaaatgat
401   tcactccata gtctgataga tcttttcaag aattgattaa tcggacgaga
451   ataaagatag agtcccgttc tacatgtcaa tgtcggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details