JRC GMO-Amplicons | EURL GMFF

Record ID:	FN386350.1
Database:	nt	Go to original record
Description:	Pyrostria serpentina trnT-trnL IGS(partial), tRNA-Leu gene and trnL-trnF IGS(partial), specimen voucher K:Davis 1162
Taxon:	Pyrostria serpentina
Original size:	1651 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	785	1335	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aggggcaatc ctgagccaaa tcctgttttc cgaaaccaaa ggttcagaaa
151   gtgaaaaagg gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catcgaaaat tccgaaagga
251   taaaacgtat acgtattcaa tactatatca aatgattaat gacgactcga
301   ctgaatctgt attttttata tgaaaatgga agaattggtg tgaatagatt
351   ccacattgaa gaaagaatcg aatattcatt gattaaatga ttcactccat
401   agtctgatag atcttttcaa gaattgatta atcggacgag aataaagata
451   gagtcccgtt ctacatgtca atgtcggcaa caatgaaatt tatagtaata
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details