JRC GMO-Amplicons | EURL GMFF

Record ID:	FN386339.1
Database:	nt	Go to original record
Description:	Pseudopeponidium sp. 1 Karehed et al. 222 trnT-trnL IGS(partial), tRNA-Leu gene and trnL-trnF IGS(partial), specimen voucher UPS:Karehed et al. 222
Taxon:	Pseudopeponidium sp. 1 Karehed et al. 222
Original size:	1653 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	778	1336	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   taataaaaag gggcaatcct gagccaaatc ctgttttccg aaaccaaagg
151   ttcagaaagt gaaaaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgcg ttagtagaga aatctttcca tcgaaaattc
251   cgaaaggata aaacgtataa gtattgaata ctatatcaaa tgattaatga
301   cgactcgact gaatctgtat tttttatatg aaaatggaag aattggtgtg
351   aatagattcc acattgaaga aagaatcgaa tattcattga ttaaatgatt
401   cactccatag tctgatagat cttttcaaga attgattaat cggacgagaa
451   taaagataga gtcccgttct acatggcaat gtcggcaaca atgaaattta
501   tagtaatagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details