JRC GMO-Amplicons | EURL GMFF

Record ID:	FN386330.1
Database:	nt	Go to original record
Description:	Peponidium madagascariense trnT-trnL IGS(partial), tRNA-Leu gene, and trnL-trnF IGS (partial), specimen voucher TAN:Razafimandimbison 431
Taxon:	Peponidium madagascariense
Original size:	1656 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	796	1339	+	544 bp

Amplicon:

544 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactatatc aaataattaa tgacgactcg
301   actgaatctg ttttttttat atgaaaatgg aagaattggt gtgaatagat
351   tccacattga agaaagattc attgattaaa tgattcactc catagtctga
401   tagatctttt caagaattga ttaatcggac gagaataaag atagagtccc
451   gttctacatg tcaatgtcgg caacaatgaa atttatagta ataggaaaat
501   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	544	525	2.45e-08

Match details