JRC GMO-Amplicons | EURL GMFF

Record ID:	FN386319.1
Database:	nt	Go to original record
Description:	Canthium mombazense trnT-trnL IGS(partial), tRNA-Leu gene, and trnL-trnF IGS (partial), specimen voucher S:Mwangoka et al. 3375
Taxon:	Bullockia mombazensis
Original size:	1647 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	784	1334	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa tcctgttttc cgaaaccgaa ggttcagaaa
151   gtgaaaaagg gataagtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catcgaaaat tccgaaagga
251   taaaacgtat acntattgaa tactatatca aatgattaat gacgactcna
301   ctgaatctgt attttttata tgaaaatgga agaattggtg tgaatagatt
351   ccacattgaa naaagaatcg aatattcatt gattaaatga tccactccat
401   agtctgatag atcttttcaa gaattgatta atcggacgag aataaagata
451   gagtcccgtt ttacatgtca atgtcggcaa caatgaaatt tatagtaata
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details