JRC GMO-Amplicons | EURL GMFF

Record ID:	FN376357.1
Database:	nt	Go to original record
Description:	Rhodopentas bussei partial trnL gene and trnL-trnF IGS, specimen voucher UPS:Luke X9311
Taxon:	Rhodopentas bussei
Original size:	1660 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	719	1303	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcggaa
151   agtgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcggtagtc gagaagcctt ttcattcatc aaaaattcat
251   aaagtctaaa aagatgaagt gaaggataaa cgtatatcca tacgtagtaa
301   atagtatatt aaatgagtaa tgacagcccg aatgaatcta tatttttttc
351   tatgaacaaa taaagaattg ttgttagtag attccgcgtt taagaaagaa
401   tcaaatattc attgatcaaa tgattcactc catagtctga tcgatctttt
451   caccaactga ttaatcggac gagaataaag atagagtccc attctacatg
501   tcaatgccgg caacaatgaa atttatagtt aagaggaaaa tccgtcgact
551   tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details