JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ324462.1
Database:	nt	Go to original record
Description:	Poa trivialis voucher US:Soreng 4681-1 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Poa trivialis
Original size:	1766 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	848	1466	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaataagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttcgaaa
251   ttctaaaaag aagggcttta tacagctaat aaacacgtat agatactgag
301   atagcaaacg attaatcaca gaacccatat tataatatta taatattggt
351   tctttattct tttttagaat gaaatagaaa tgattatgaa ataaaaaatt
401   cataattttt ttagaattat tttgaatcca ttccaatcga atatttagta
451   atcaaatcct tcaattcaag gttttgaggt ctttaaaaaa gtggattaat
501   cggacgagga caaagagaga gtcccattct acatgtcaat actgacaaca
551   atgaaatttc tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details