JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ324451.1
Database:	nt	Go to original record
Description:	Poa remota voucher US:Stoneberg PB3-A trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Poa remota
Original size:	1802 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	841	1446	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttcgaaa
251   ttctaaaaag aagggcttta tacagctaat aaacacgtat agatactgag
301   atagcaaacg attaatcaca gagcccatat tataatataa tattggttct
351   ttattctttt ttagaatgaa atttgaaata aaaaattcat aattttttta
401   gaattattgt gaatccattc caatcgaata tttagtaatc aaatccttca
451   attcaaagtt ttgaggtctt taaaaaagtg gattaatcgg acgaggacaa
501   agagagagtc ccattctaca tgtcaatact gacaacaatg aaatttctag
551   taaaaggaaa atccgtcgac tttataagtc gtgagggttc aagtccctct
601   atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details