JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ324433.1
Database:	nt	Go to original record
Description:	Poa lowanensis voucher MEL:Walsh s.n. 2323766 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Poa lowanensis
Original size:	1812 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	839	1456	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttcgaaa
251   ttctaaaaag aagggcttta tacagctaat aaacacgtat agatactgag
301   atagcaaacg attaatcaca gagcccatat tataatattg gttctttatt
351   cttttttaga atgaaatttg aaatagaaat gattatgaaa taaaaaattc
401   ataatttttt tagaattatt gtgaatccat tccaatcgaa tatttagtaa
451   tcaaatcctt cagttcaaag ttttgagatc tttaaaaaag tggattaatc
501   ggacgaggac aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttct agtaaaagga aaatccgtcg actttataag tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details