JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492309.1
Database:	nt	Go to original record
Description:	Sarmienta scandens chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Sarmienta scandens
Original size:	971 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	561	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa agattcagaa
151   agcgaaaaag gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatatt atatcaaatg attaataacg
301   gcccgaatct gtatctgtat ttttgtatat gaaaaatgga agaattggtg
351   tgaattgatt ccacattgaa gaaagaatca aatattcatt catcaaataa
401   ttcactccat agtccgatag atcttttaaa gaacttcaaa atcggacgag
451   aataaagata gagtcccgtt ctacatgtca ataccggcaa caatgaaact
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details