JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492304.1
Database:	nt	Go to original record
Description:	Streptocarpus holstii chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Streptocarpus holstii
Original size:	946 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	546	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agggttcaga
151   gagcgaaaaa vgaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatt gaaacttcag aaaggatgaa
251   acgtatctat tgaatactat tgatcattaa taatggccca catctgtatc
301   tgggtttttg tatatgaaaa atggaagaat tggtgtgaat tgatttcaca
351   ttgataaaag aatcgaatat tcattgatca aatcattgac ccgatagtcc
401   gatagatctc ttaaagaatt gattattcgg acgagaataa agatagagtc
451   ccgttctaca tgtcaatacc gacaacaatg aaacttatag taagaggaaa
501   atccgtcgac ttttaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details