JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492302.1
Database:	nt	Go to original record
Description:	Rhabdothamnopsis sinensis chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Rhabdothamnopsis sinensis
Original size:	957 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	553	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt atcaaaacaa gggttcagag
151   ggcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tacgttggta gaggaattga aactttagaa agaatgaaag
251   ataaacgtat ctattgaata ttattgatga ttaataatgg cccgaatctg
301   tatctgggtt tttgtatatg aaagaaaaat ggaagaattg gtgttaattg
351   atttcacatt gataaaagaa tcaaatattc attgatcaaa tcattcactc
401   cacagtccga tagatctctt aaagaactta ttattcggac gagaataaag
451   ataggtcccg ttctacatgt caataccggc aacaatgaaa cttatagtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details