JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492299.1
Database:	nt	Go to original record
Description:	Petrocosmea nervosa chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Petrocosmea nervosa
Original size:	939 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	543	+	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa ggattcagag
151   agcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac cgcgttggta gacgaattga aacttcagaa aggataaacg
251   tatctattga atactattta tgattaataa tggtccgaat ctgtatctgg
301   gtttttgtat ataaaaaata gaagaattgg tgtgaattga tttcacattg
351   ataaaagaat cgaatattaa ttaatcaaat cattcactcc acagtccgat
401   agatctctta aagaacttat tattcggacg agaataaaga tagagtcccg
451   ttctacatgt caataccggc aacaatgaaa cttatagtaa gaggaaaatc
501   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details