JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492269.1
Database:	nt	Go to original record
Description:	Monophyllaea horsfieldii chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Monophyllaea horsfieldii
Original size:	970 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	569	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   aatgggcaat cctgagccaa atcccgtttt ctcaaaacaa aggttcagag
151   ggcgaaaaag aggataggtg cagagactca atggagggtg ttctaacaaa
201   tggagttgac tgcgttggga gaggaatcgt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact aatatcaata agattaataa
301   tggcccgatt ctgtatctgg atttttgtat atgaaaaacg gaagaattta
351   tgtgaattga ttccacattg aagaaaaaat cgaatagtca ttcatcaaca
401   tcaaattatt cactccaaag tccgagagat cttttaaaga acttattaag
451   cggacgagaa taaagataga gtcccgttct acatgtcaat accggcaaca
501   atgaaactta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details