JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492296.1
Database:	nt	Go to original record
Description:	Haberlea rhodopensis chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Haberlea rhodopensis
Original size:	959 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	550	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa gggttcagag
151   agcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatcga aactttagaa aggatgaagg
251   ataaacgtat ctattgaata ctattgatga ttaataatgg cccgaatctg
301   tatctgggtt tttgtatatg aaaaatggaa taattggtgt gaattgattt
351   cacattgata aaagaatcga atattcattg atcaaatcat tcactccata
401   gtccgataga tctcttaaag aacttattat tcggacgaga ataaagatag
451   agtcccgttc tacatgtcaa taccggcaac aatgaaactt atagtaaaag
501   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details