JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492294.1
Database:	nt	Go to original record
Description:	Didymocarpus cordatus chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Didymocarpus cordatus
Original size:	947 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	549	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa gggttcagag
151   agcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac cgcgttggta gaaaattgaa acttcagaaa ggatgaagga
251   taaacgtatc tattgaatac tattgatgat taataatggc ccgaatccat
301   atctgggttt ttgtatatga aaaatagaag aattggtgtg aattgatttc
351   acattgataa aagaatcgaa tattcattaa tcaaatcatt cactccacag
401   tccgatagat ctcttaaaga acttattatt cggacgagaa taaagataga
451   gtcccgttct acatgtcaat accggcaaca atgaaactta tagtaagagg
501   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details