JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492291.1
Database:	nt	Go to original record
Description:	Chirita spadiciformis chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Primulina spadiciformis
Original size:	948 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	552	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt cagattcaga gaaaccctgg aattaataaa
101   aatgggcaat cgtgagccaa atccttgttt tctcaaaaca agggttcaga
151   gagcgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ccccgctggt agaagaattg aaacttcaga aaggatgaag
251   gataaacgta tctattgaat actattgatg attaataatg gcccgaatct
301   gtatctgggt atttcgtata tgaaaaatag aagaattggt gtgaattgat
351   ttcacattga taaaagaatc gaatatttat taatcaaatc attcactcca
401   cagtccgata gatctcttaa agaacttatt attcggacga gaataaagat
451   agagtcccgt tctacatgtc aataccggca acaatgaaac ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details