Match details
Record ID: |
EF440565.1 |
Database: |
nt |
Go to original record |
Description: |
Picea sitchensis from USA tRNA-Thr (trnT) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast. |
Taxon: |
Picea sitchensis |
Original size: |
1435 bp |
View other 20492 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
446 |
1007 |
+ |
562 bp |
|
1 cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51 acctaccaag tgatagcttc caaatccagg gaaccctggg atattttgaa
101 tgggtaatcc tgagccaaat ccggttcatt gagacaatag tttcttcttt
151 tcttctccta agataggaag gggataggtg cagagactca atggaagcta
201 ttctaacgaa tgaatctcat ttggtccaat actgtattta tagaacgctc
251 tatttacacc taaaaagtgg gaatgtgata taacatcaga caaaactcgc
301 gatcagaact tgaatcgttc caagcatcta ttcgtaagat agatgccaga
351 ttcgagttga agtactgatt tgacattaag taatccaatt atgaacttct
401 mtactttaga tagagaattg aatcagtttt tggaataaat ggttggacga
451 gaataaagat agagtccaat tctacgtgtc aatgtcaaca acaatgcaaa
501 ttgcagtagg aggaaaatcc gttggcttta tagaccgtga gggttcaagt
551 ccctctatcc cc
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
20 bp |
0 |
0 |
1 |
20 |
2.53e-08 |
primerB |
100.0 |
20 bp |
0 |
0 |
562 |
543 |
2.53e-08 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.