JRC GMO-Amplicons | EURL GMFF

Record ID:	EF440564.1
Database:	nt	Go to original record
Description:	Picea schrenkiana from China tRNA-Thr (trnT) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Picea schrenkiana
Original size:	1423 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	446	1001	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaatccagg gaaccctggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcatg gagacaatag tttcttcttt
151   tattctccta agataggaag gggataggtg cagagactca atggaagcta
201   ttctaacgaa tgaatctcat ttggtccaat actgtattta tagaacgctc
251   tatttacacc taaaaagtgg gaatgtgata taacatcaga caaaactcgc
301   gatcagaact tgaatcgttc caagcatcta ttcgagatgc cagattcgag
351   ttgaagtact gattttacat taagtaatcc aattatgaac ttctctactt
401   tagatagaga attgaatcag tttttggaat aaatggttgg acgagaataa
451   agatagagtc caattctacg tgtcaatgtc aacaacaatg caaattgcag
501   taggaggaaa atccgttggc tttatagacc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details