JRC GMO-Amplicons | EURL GMFF

Record ID:	EF440558.1
Database:	nt	Go to original record
Description:	Pinus banksiana tRNA-Thr (trnT) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Pinus banksiana
Original size:	1468 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	478	1037	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaatccagg gaaccctggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcatg aagacaatgt ttcttctcct
151   aatataggaa ggggataggt gcagagactc aatggaagct attctaacga
201   atgaatctca tttggtccaa tactgtagtt atagaacgat ctatttacac
251   ctcaaaaatg gagagagaga tgttatataa caccagacaa aactggcgat
301   cagaacttga atcgttccaa gcattttatt cataagatag atgccagatt
351   caagttgaag tactgatttg acattaagta atccaattat gaacttatct
401   actctagatg gataatttaa ttcttttttg gaataaatgg ttggacgagg
451   ataaagatag agtccaattc tacatgtcaa tgtcaacaac aatgcaaatt
501   gcagtaggag gaaaatccgt tggctttata gaccgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details