JRC GMO-Amplicons | EURL GMFF

Record ID:	GU594576.1
Database:	nt	Go to original record
Description:	Moorochloa malacodes tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Moorochloa malacodes
Original size:	929 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	622	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctgttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   tatagaaaga agggctttat acatctaata cacacgtata agatactgac
301   atagcaaacg attaatcacg gaacccatat aataatatag gttctttatt
351   ttatattttt ttttttagaa tgaaattagg aatgattatg aaatagaaaa
401   ttctgaattt tttgagaatt attgtgaatc cattccaatc gaatattgag
451   taatcaaatc cttcaattca ttgttttgag atatttaaaa aagtggatta
501   atcgaacgag gataaagaga gagtcccatt ctacatgtca atactgacaa
551   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details