JRC GMO-Amplicons | EURL GMFF

Record ID:	GU594571.1
Database:	nt	Go to original record
Description:	Brachiaria deflexa tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Urochloa deflexa
Original size:	940 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	619	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgttaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctcttttga aaaaagaagt gtttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact cccttgaaat
251   tatagaaaga agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacag aacccatatc ataatatagg ttctttattt
351   tatatttttt tttagaatga aatttggaat gattatgaaa tagaaaattc
401   tgaatttttt gagaattatt gtgaattcat tccaatcgaa tattgagtaa
451   tcaaatcctt caactcattg ttttgagatc tttaaaaaag tggattaatc
501   gaacgaggat aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttct agtaaaagga aaatccgtcg actttataag tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details