JRC GMO-Amplicons | EURL GMFF

Record ID:	GU594566.1
Database:	nt	Go to original record
Description:	Urochloa subquadripara tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Urochloa subquadripara
Original size:	934 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	618	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctcttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   tatagaaaga ggggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatccaga acccatatca taatataggt tctttatttt
351   atattttttt ttagaatgaa attaggaatg ataatgaaat agaaaattct
401   gaattttttg agaattattg tgaatccatt ccaatcgaat attgagtaat
451   caaatccttc aactcattat tttgagatct ttaaaaaagt ggattaatcg
501   aacgaggata aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details