JRC GMO-Amplicons | EURL GMFF

Record ID:	GU594565.1
Database:	nt	Go to original record
Description:	Urochloa subulifolia tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Urochloa subulifolia
Original size:	951 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	621	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctcttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   tatagaaaga agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatcaaag aacccatatc ataatatagg ttctttattt
351   tatctttttt tttttagaat taaattagga atgattatga aatagaaaat
401   tctgaatttt ttgagaatta ttgtgaatcc attccaatcg aatattgagt
451   aatcaaatcc ttcaactcat tgttttgaga tctttaaaaa agtggattaa
501   tcgaacgagg ataaagagag agtcccattc tacatgtcaa tactgacaac
551   aatgaaattt ctagtaaaag gaaaatccgt cgactttata agtcgtgagg
601   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details