JRC GMO-Amplicons | EURL GMFF

Record ID:	GU594561.1
Database:	nt	Go to original record
Description:	Melinis repens tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Melinis repens
Original size:	928 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	620	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaattacg ttgtgttggt agtggaactc cctcgaaatt
251   atagaaagaa gggctttata catctaatac acacatagat actgacatag
301   caaacgatta atcacagaac ccatatcata atataggttc cttattttat
351   atttttttta gaatgaaatt aggaatgatt atgaaataga aaattctgaa
401   ttttttgaga attattgtga atccattcca ttccaatcga atattgagta
451   atcaaatcct tcaattcatt gttttgagat atttttaaaa atggattaat
501   cgaacgagga taaagagaga gtcccattct acatgtcaat actgacaaca
551   atgaaatttc tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details