JRC GMO-Amplicons | EURL GMFF

Record ID:	GU594545.1
Database:	nt	Go to original record
Description:	Urochloa lorentziana tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Urochloa lorentziana
Original size:	931 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	610	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   tatagaaaga agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacag aacccatagg ttctttattt tatatttttt
351   ttttagaatg aaattaggaa tgattatgaa atagaaaatt ctgaattttt
401   ttagaattat tgtgaatcca ttccaatcga atattgagta atcaaatcct
451   tcaattcatt gttttgagat ctttaaaaaa gtggattaat cgaacgagga
501   taaagagaga gtcccattct acatgtcaat actgacaaca atgaaatttc
551   tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details