JRC GMO-Amplicons | EURL GMFF

Record ID:	GU594543.1
Database:	nt	Go to original record
Description:	Urochloa ruziziensis tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Urochloa ruziziensis
Original size:	934 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	611	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctcttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg cagtggaact ccatagaaag
251   aagggcttta tacatctaat acacacgtat agatactgac atagcaaacg
301   attaatcaca gaacccatat cataatatag gttctttatt ttctattttt
351   tttttagaat gaaattagga atgattatga aatagaaaat tcataatttt
401   ttgagaatta ttgtgaatcc attccaatcg aatattgagt aatcaaatcc
451   ttcaactcat tgttttgaga tctttaaaaa agtggattaa tcgaacgagg
501   ataaagagag agtcccattc tacatgtcaa tactgacaac aatgaaattt
551   ctagtaaaag gaaaatccgt cgactttata agtcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details