JRC GMO-Amplicons | EURL GMFF

Record ID:	GU594533.1
Database:	nt	Go to original record
Description:	Urochloa plantaginea tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Urochloa plantaginea
Original size:	906 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	617	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctcttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   tatagaaaga agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatccaga acccatatca taatataggt tctttatttt
351   agattttttt tagaatgaaa ttaggaatga ttatgaaata gaaaattctg
401   aattttttga gaattattgt gaatccattc caatcgaata ttgagtaatc
451   aaatccttca actcattatt ttgagatctt taaaaaagtg gattaatcga
501   acgaggataa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttataagtc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details