JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ346953.1
Database:	nt	Go to original record
Description:	Uncaria sp. Harder et al. 4242 chloroplast DNA for trnT-L-F region, specimen voucher Harder et al. 4242 (MO)
Taxon:	Uncaria sp. Harder et al. 4242
Original size:	1724 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	743	1311	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    actcactaag tgataacttt caaattcaga gaaacccgga attaataaaa
101   agggcaatcc tgagccaaat cctgttttcc gaaaccaaag gttcagaaag
151   tganaaaggg ataggtgcag agactcaacg gaagctgttc taacaaatgg
201   agttggatgc attagtagag agatctttcc atcgaaaatt cagaaagaat
251   gaagtgaagg ataaacgtat atacatacgt attgaatact atatcaaatg
301   attaatgacg acccgactga atctgtattt atatatatga aaaacggaag
351   aattggtgtg aatagattcc acattgaaga aagaatcgaa tattcattga
401   tcaaatgatt cactccatag tctgatagat cttttcaaga actgattaat
451   cggacgagaa taaagataga gtcccattct acatgtcaat gccggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details