JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ346946.1
Database:	nt	Go to original record
Description:	Pausinystalia lane-poolei subsp. lane-poolei chloroplast DNA for trnT-L-F region, specimen voucher Hart 1350 (BR)
Taxon:	Corynanthe lane-poolei subsp. lane-poolei
Original size:	1734 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	751	1321	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttagat gcattagtag agagatcttt ccatcgaaaa ttcagaaaga
251   atgaagtgaa ggataaacgt atatacatac gtattgaata ctatatcaaa
301   tgattaatga cgacccgact gaatctgtat ttttatatat gaaaaacgga
351   agaattggtg tgaatagatt ccacattgaa gaaagaatcg aatattcatt
401   gatcaaatga ttcactccat agtctgatag atcttttcaa gaactgatta
451   atcggacgag aataaagata gagtcccatt ctacatgtca atgccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details