JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ346941.1
Database:	nt	Go to original record
Description:	Neonauclea forsteri chloroplast DNA for trnT-L-F region, specimen voucher Lorence 8668 (NTBG)
Taxon:	Neonauclea forsteri
Original size:	1732 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	749	1319	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaatnaa
101   aaagggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttagat gcattagtag agagatcttt ccatcgaaaa ttcagaaaga
251   atgaagtgaa agataaacgt atatacatac gtattgaata ctatatcaaa
301   tgattaataa cgacccgact gaatctgtat ttttatatat gaaaaacgga
351   agaattggtg tgaatagatt ccacattgaa gaaagaatcg aatattcatt
401   gatcaaatga ttcactccat agtctgatag atcttttcaa gaactgatta
451   atcggacgag aataaagata gagtcccatt ctacatgtca atgccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details